Motor Proteins: Kinesin Drives with an Underhead Cam
نویسندگان
چکیده
A high-resolution cryo-EM structure of kinesin bound to its microtubule track allows for near-atomistic visualization of nucleotide-dependent conformational changes in this motor protein.
منابع مشابه
Targeted disruption of Ca2+-calmodulin signaling in Drosophila growth cones leads to stalls in axon extension and errors in axon guidance
Ca(2+)-calmodulin (CaM) function was selectively disrupted in a specific subset of growth cones in transgenic Drosophila embryos in which a specific enhancer element drives the expression of the kinesin motor domain fused to a CaM antagonist peptide (kinesin-antagonist or KA, which blocks CaM binding to target proteins) or CaM itself (kinesin-CaM or KC, which acts as a Ca(2+)-binding protein). ...
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Plant kinesin-like calmodulin-binding protein (KCBP) is a novel member of the kinesin superfamily that interacts with calmodulin (CaM) via its CaM-binding domain (CBD). Activated CaM (Ca(2+)-CaM) has been shown to inhibit KCBP interaction with microtubules (MTs) thereby abolishing its motor- and MT-dependent ATPase activities. To test whether the fusion of CBD to non-CaM-binding kinesins confer...
متن کاملThe Calmodulin-binding Domain from a Plant Kinesin Functions as a Modular Domain in Conferring Ca -Calmodulin Regulation to Animal Plus- and Minus-end Kinesins*
Plant kinesin-like calmodulin-binding protein (KCBP) is a novel member of the kinesin superfamily that interacts with calmodulin (CaM) via its CaM-binding domain (CBD). Activated CaM (Ca -CaM) has been shown to inhibit KCBP interaction with microtubules (MTs) thereby abolishing its motorand MT-dependent ATPase activities. To test whether the fusion of CBD to non-CaM-binding kinesins confers Ca ...
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KCBP (kinesin-like calmodulin [CaM]-binding proteins), a member of the carboxy-terminal kinesin-like proteins (KLPs), is unique among KLPs in having a CaM-binding domain (CBD). CaM-binding KLPs have been identified from flowering plants and the sea urchin. To determine if CaM-binding KLP is present in phylogenetically divergent protists, we probed Cyanophora paradoxa protein extract with affini...
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Inside the cell, vital processes such as cell division and intracellular transport are driven by the concerted action of different molecular motor proteins. In C. elegans chemosensory cilia, 2 kinesin-2 family motor proteins, kinesin-II and OSM-3, team up to drive intraflagellar transport (IFT) in the anterograde direction, from base to tip, whereas IFT dynein hitchhikes toward the tip and subs...
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عنوان ژورنال:
- Current Biology
دوره 20 شماره
صفحات -
تاریخ انتشار 2010